In Vitro Production of Mouse Mammary Tumor Virus in a Mouse Mammary Tumor Ascites Line
نویسندگان
چکیده
منابع مشابه
In vitro production of mouse mammary tumor virus in a mouse mammary tumor ascites line.
An ascites line derived from a spontaneous mouse mammary carcinoma produces, on explantation and cultivation in vitro, large amounts of oncornavirus particles. The biochemical, biophysical, and electron microscopic characteristics of the virions are described. Molecular hybridization and immunological methods identify these virions as mouse mammary tumor virus.
متن کاملStimulation of mammary tumor virus production in a mouse mammary tumor cell line.
SUMMARY A mouse mammary tumor cell line (MMT), which has shown since its isolation in 1962 a limited, but continuous, budding of B particles, was stimulated to high production by alternate passages of the cells through mice or rats and tissue culture. Newborn C57BL, Af, and A mice and Amster dam/IMR rats have been used as transient hosts. Detection and semiquantitative estimations of virus prod...
متن کاملUltrastructural and quantitative studies of mammary tumor virus production in cultured mouse mammary tumor cells.
A whole-cell-mounting technique was successfully applied to the study of mouse mammary tumor virus budding from cells in tissue culture. The technique permitted the visualiza tion of ultrastructural detail, including the cores and the surface spikes of budding virions. The latter are difficult to discriminate in thin sections. Budding mammary tumor virus was compared with budding murine leukemi...
متن کاملUltrastructural and Quantitative Studies of Mammary Tumor Virus Production in Cultured Mouse Mammary Tumor Cells
A whole-cell-mounting technique was successfully applied to the study of mouse mammary tumor virus budding from cells in tissue culture. The technique permitted the visualiza tion of ultrastructural detail, including the cores and the surface spikes of budding virions. The latter are difficult to discriminate in thin sections. Budding mammary tumor virus was compared with budding murine leukemi...
متن کاملCryoelectron microscopy of mouse mammary tumor virus.
Cryoelectron microscopy of Mouse mammary tumor virus, a Betaretrovirus, provided information about glycoprotein structure and core formation. The virions showed the broad range of diameters typical of retroviruses. Betaretroviruses assemble cytoplasmically, so the broad size range cannot reflect the use of the plasma membrane as a platform for assembly.
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1973
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.70.10.2983